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Image Search Results
Journal: Protein Science : A Publication of the Protein Society
Article Title: Oligomerization of protein arginine methyltransferase 1 and its effect on methyltransferase activity and substrate specificity
doi: 10.1002/pro.5118
Figure Lengend Snippet: Cross‐linking of peptide substrate to wild‐type (WT) PRMT1 (tetramer), tetramer interface mutant (TIM)2 PRMT1 (dimer), and dimer interface mutant (DIM) PRMT1 (monomer). The biotinylated H421 peptide substrate was crosslinked to WT, TIM2, or DIM PRMT1 using glutaraldehyde and samples were separated using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride. The blot was probed for biotin using StrepTactin‐HRP. Protein standards (STDS) were imaged colorimetrically.
Article Snippet: The membrane was probed with 1:5000
Techniques: Mutagenesis, Polyacrylamide Gel Electrophoresis